The Structural Genomics Consortium (SGC) is a milestone-driven research enterprise that develops and employs both novel and established High Throughput methodologies to meet aggressive antibody selection and production output goals.
Laboratory members work in a team matrix structure with a quick-paced environment.
In this position, the technician will work with collaborators in another lab at the University of Toronto, as well as SGC Scientists, to develop and employ both novel and established high throughput methodologies to meet aggressive milestones.
As part of an epigenetics program, the incumbent will validate antibodies in cell-based assays to different protein families involved in epigenetic signaling including the Histone Acetyltransferases, Histone Methyltransferases, and other methyl lysine binding modules. Responsibilities will include: Screening antibody candidates in Fab and IgG format in various cell-based assays and applications, mammalian cell culture to prepare assay material needed, recording all experimental details and organize related data in an electronic lab notebook, entering data in SGC databases where applicable, and preparing progress reports and contributing to writing related publications.
Working under the general supervision of the Biotechnology Group Leader, the laboratory technician will execute experimental strategies and workflows, conduct experiments, interpret experimental data, and identify and suggest solutions to problems. In addition, SGC laboratory technicians contribute towards the establishment and maintenance of a safe and productive laboratory work environment.
Bachelor of Science degree; or acceptable equivalent combination of education and work experience in biochemistry or related majors. Area of Specialization: Molecular Biology, Chromatin Biology and Epigenetics, DNA damage and Cancer genetics, Cancer Biology.
A minimum of 2 years of relevant experience in antibody validation is essential. Experience with high throughput biotechnology platforms is also required. This position requires hands-on experience in molecular biology techniques including immunoprecipitation, western blotting and mass spectrometry. A unique blend of knowledge in protein characterization, antibody validation and mammalian cell culture is required.
Familiarity with different protein purification methods. Ability to validate antibodies or antibody-like Molecules; Western Blot (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Immunoflourescence (IF) and IP-Mass Spectrometry (IP-MS). Ability to perform mammalian cell culture Ability to work within a team and deliver according to deadlines and milestones. Strong analytical thinking and attention to detail are essential. Other experiences include superior communication skills and ability to collaborate and work as a part of a group.
United Steelworkers (USW)
Grant - Continuing
Pay Scale Group and Hiring Rate
09 -- $50,965 with an annual step progression to a maximum of $65,176. Pay Rates effective July 1, 2012 under the Job Evaluation/Pay Equity Maintenance Protocol.